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isotype control rat igg 2b antibody  (Bio X Cell)


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    Bio X Cell isotype control rat igg 2b antibody
    Isotype Control Rat Igg 2b Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+igg+2b+antibody/anti+cd4+gk1+5/pm38580335-58-15-20
    Average 90 stars, based on 1 article reviews
    isotype control rat igg 2b antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Control:

    Article Title: Targeting PARG induces tumor cell growth inhibition and antitumor immune response by reducing phosphorylated STAT3 in ovarian cancer
    Article Snippet: .. In this study, we used anti-mouse CD8α (clone 53-6.72) monoclonal antibody (mAb) and isotype control rat IgG 2b (BioXcell) for in vivo depletion of CD8 + T cell experiments. ..

    Article Title: Repeated social defeat stress differently affects arthritis-associated hypersensitivity in male and female mice.
    Article Snippet: The anesthesia was antagonized with i.p. injection of Naloxone (0.4 mg/kg; Inresa Arzneimittel, Germany), Flumazenil (0.5 mg/kg; Fresenius, Germany), and Atipamezole (2.5 mg/kg; Prodivet Pharmaceuticals, Belgium). .. Male C57BL/6J mice with K/C-induced arthritis received daily i.p. injections of a rat anti-mouse C-C motif chemokine receptor (CCR2) monoclonal antibody (clone MC-21, provided by Prof. Matthias Mack), or IgG2b kappa isotype control (clone LTF-2, BioXCell, Germany) (Brühl et al., 2007; Mack et al., 2001; Pfau et al., 2019). ..

    Article Title: Targeting PARG induces tumor cell growth inhibition and antitumor immune response by reducing phosphorylated STAT3 in ovarian cancer.
    Article Snippet: .. In this study, we used anti- mouse CD8α (clone 53- 6.72) monoclonal antibody (mAb) and isotype control rat IgG 2b (BioXcell) for in vivo depletion of CD8+ T cell experiments. ..

    In Vivo:

    Article Title: Targeting PARG induces tumor cell growth inhibition and antitumor immune response by reducing phosphorylated STAT3 in ovarian cancer
    Article Snippet: .. In this study, we used anti-mouse CD8α (clone 53-6.72) monoclonal antibody (mAb) and isotype control rat IgG 2b (BioXcell) for in vivo depletion of CD8 + T cell experiments. ..

    Article Title: PD-1/CD80 + small extracellular vesicles from immunocytes induce cold tumours featured with enhanced adaptive immunosuppression
    Article Snippet: .. Antibodies used for in vivo PD-1 blockade experiments were given intraperitoneally at a dose of 200 μg per mouse and include: PD-1 (RMP1-14) and rat IgG 2b isotype (LTF-2) (BioXCell). ..

    Article Title: Targeting PARG induces tumor cell growth inhibition and antitumor immune response by reducing phosphorylated STAT3 in ovarian cancer.
    Article Snippet: .. In this study, we used anti- mouse CD8α (clone 53- 6.72) monoclonal antibody (mAb) and isotype control rat IgG 2b (BioXcell) for in vivo depletion of CD8+ T cell experiments. ..

    Injection:

    Article Title: Administration of sting agonist and checkpoint inhibitors
    Article Snippet: .. Anti-mouse PD1 antibody (anti-mPD-1) (Bio X Cell, 10 Technology Drive, Suite 2B, West Lebanon, NH 03784) was formulated prior to each injection at 2 mg/mL in Phosphate-buffered saline (PBS) and administered intraperitoneally (IP) based on exact body weight on each day of treatment, using a dosing volume of 5 mL/kg resulting in a 10 mg/kg dose. ..

    Saline:

    Article Title: Administration of sting agonist and checkpoint inhibitors
    Article Snippet: .. Anti-mouse PD1 antibody (anti-mPD-1) (Bio X Cell, 10 Technology Drive, Suite 2B, West Lebanon, NH 03784) was formulated prior to each injection at 2 mg/mL in Phosphate-buffered saline (PBS) and administered intraperitoneally (IP) based on exact body weight on each day of treatment, using a dosing volume of 5 mL/kg resulting in a 10 mg/kg dose. ..

    Incubation:

    Article Title: Imlifidase and EndoS enables semi-allogeneic bone marrow engraftment in sensitized mice under reduced intensity conditioning
    Article Snippet: .. Rag2p GFP F1 splenocytes (2 × 10 5 ) were treated with FcR blockade (anti-mouse CD16/CD32 rat IgG 2b antibodies, clone 2.4G2, BE0307, Bio X cell) for 5 minutes, followed by incubation with a titrated quantity of sera in 100μL for 30 minutes. ..



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    Expression and localization of marker proteins in hiPSC-derived brain cell types of the NVU. HiPSCs were derived from a late-onset Alzheimer disease patient (LOAD NVU model) and a healthy elderly control subject (CON NVU model). ( A ) Flow cytometry confirmed high expression of cell type-specific phenotypic markers in pericytes (PCs), astrocytes (ACs), neural stem cells (NSCs; all mean ± SD; n ≥ 3), and microglia-like cells (MGCs; mean ± SD; n = 2). Representative immunofluorescence images confirmed protein expression of ( B ) NG2 and αSMA in PCs, scale bar 200 μm, ( C ) EAAT1 and EAAT2 in ACs, scale bar 100 μm, ( D ) CD45 and AIF1 in MGCs, scale bar 100 μm, ( E ) PAX6, SOX1, and NES in NSCs, scale bar 100 μm, and ( F ) GLUT1/SLC2A1, ZO1, OCLN, and CLDN5 in BCECs, scale bar 100 μm. ( G ) Measurement of transendothelial electrical resistance (TEER) and sodium fluorescein (NaF) permeability to analyze the integrity of the blood-brain barrier in CON and LOAD. TEER ( left ) and permeability coefficient (PC NaF ) ( right ) values are shown for mono- and co-cultures consisting of BCECs with or without PCs, ACs, MGCs, or NSCs. Box (median and lower/upper quartile) and whisker (minimum/maximum) plots display n = 4–6 independent biological assays (no outliers), one-tailed t-test, *p < 0.05

    Journal: Fluids and Barriers of the CNS

    Article Title: Human isogenic cells of the neurovascular unit exert transcriptomic cell type-specific effects on a blood-brain barrier in vitro model of late-onset Alzheimer disease

    doi: 10.1186/s12987-023-00471-y

    Figure Lengend Snippet: Expression and localization of marker proteins in hiPSC-derived brain cell types of the NVU. HiPSCs were derived from a late-onset Alzheimer disease patient (LOAD NVU model) and a healthy elderly control subject (CON NVU model). ( A ) Flow cytometry confirmed high expression of cell type-specific phenotypic markers in pericytes (PCs), astrocytes (ACs), neural stem cells (NSCs; all mean ± SD; n ≥ 3), and microglia-like cells (MGCs; mean ± SD; n = 2). Representative immunofluorescence images confirmed protein expression of ( B ) NG2 and αSMA in PCs, scale bar 200 μm, ( C ) EAAT1 and EAAT2 in ACs, scale bar 100 μm, ( D ) CD45 and AIF1 in MGCs, scale bar 100 μm, ( E ) PAX6, SOX1, and NES in NSCs, scale bar 100 μm, and ( F ) GLUT1/SLC2A1, ZO1, OCLN, and CLDN5 in BCECs, scale bar 100 μm. ( G ) Measurement of transendothelial electrical resistance (TEER) and sodium fluorescein (NaF) permeability to analyze the integrity of the blood-brain barrier in CON and LOAD. TEER ( left ) and permeability coefficient (PC NaF ) ( right ) values are shown for mono- and co-cultures consisting of BCECs with or without PCs, ACs, MGCs, or NSCs. Box (median and lower/upper quartile) and whisker (minimum/maximum) plots display n = 4–6 independent biological assays (no outliers), one-tailed t-test, *p < 0.05

    Article Snippet: The following antibodies and dilutions were used: Mouse IgG 2a anti-human actin alpha 2, smooth muscle (ACTA2/αSMA; 1:100; #ab7817, Abcam), Goat IgG anti-human allograft inflammatory factor 1 (AIF1/IBA1; 1:200; #NB100-1028, BioTechne), Rat IgG 2b anti-human CD45 (1:200; #NB100-77417, BioTechne), Rabbit IgG anti-human claudin 5 (CLDN5; 1:100; #ab15106, Abcam), Mouse IgG 2a anti-human EAAT1/ GLAST (1:100; #sc-515839, Santa Cruz Biotechnology), Mouse IgG 2a anti-human EAAT2 (1:100; #sc-365634, Santa Cruz Biotechnology), Rabbit IgG anti-human solute carrier family 2 member 1 (SLC2A1/GLUT1; 1:200; #ab115730, Abcam), Mouse IgG 1 anti-human nestin (NES; 1:1000; #60091, Stemcell Technologies), Mouse IgG 1 anti-human chondroitin sulfate proteoglycan 4 (CSPG4/NG2; 1:100; # 83508, Abcam), Mouse IgG 1 anti-human occludin (OCLN; 1:200; #331500, Thermo Fisher Scientific), Rabbit IgG anti-human PAX6 (1:200; #ab5790, Abcam), Rabbit IgG anti-human SOX1 (1:200; #ab22572 Abcam), and Rabbit IgG anti-human tight junction protein 1 (TJP1/ZO1; 1:400; #21773-1-AP, Proteintech).

    Techniques: Expressing, Marker, Derivative Assay, Flow Cytometry, Immunofluorescence, Permeability, Whisker Assay, One-tailed Test